Abstract: Xin tian mine is located in guizhou karst development area, the karst aquifer affect mine safety production. Mine by means of controlled source audio-frequency magnetotelluric method exploration, water test, connectivity test and water the comprehensive geophysical and geochemical exploration methods such as chemical analysis found out mine embedded depth of karst development depth can reach 412.82 m, karst in the plane are controlled by topography, structure is obvious; Field boundary F1 fault for a guide water fault, is mine hydrogeological boundary, fault in mining field with hydraulic conductivity, is limestone aquifer to the mine stope filling water channel, atmospheric precipitation has a supply of aquifer, and indirectly to the stope supplies. The mine through the research on the rules of karst development, for mine offer a scientific basis for comprehensive control of karst underground water disasters.